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This Concept Map, created with IHMC CmapTools, has information related to: LAB 7 - ENDOSPORE STAIN AND BACTERIAL MOTILITY, endospores endospore stain procedure Heat-fix a smear of Bacillus megaterium to a clean glass slide., Most motile bacteria propel themselves by special organelles termed flagella. structure filament, hook, and basal body., endospore stain and bacterial motility endospores endospores, bacterial motility mechanism Most motile bacteria propel themselves by special organelles termed flagella., Place the slide over a beaker of simmering water and steam the smear in this manner for five minutes. As the malachite green evaporates, continually add more. step 4 After five minutes of steaming, wash the excess stain and blotting paper off the slide with water and blot the slide dry, endospores formation Sporulation: formation of a single endospore within a vegetative bacterium., Most motile bacteria propel themselves by special organelles termed flagella. methods of detecting motility motility test medium, Most motile bacteria propel themselves by special organelles termed flagella. flagellar arrangements axial filaments: internal flagella found only in spirochetes, Most motile bacteria propel themselves by special organelles termed flagella. flagellar arrangements amphitrichous: single flagellum at both poles, Most motile bacteria propel themselves by special organelles termed flagella. flagellar arrangements monotrichous: a single flagellum at one pole, motility test medium results non-motile: growth occurs only along the line of inoculation. Growth along the stab line is very sharp and defined., Most motile bacteria propel themselves by special organelles termed flagella. methods of detecting motility flagella staining, Sporulation: formation of a single endospore within a vegetative bacterium. germination The endospore is able to survive for long periods of time until environmental conditions again become favorable for growth. The endospore then germinates, producing a single vegetative bacterium., After five minutes of steaming, wash the excess stain and blotting paper off the slide with water and blot the slide dry step 5 Stain with safranin for 1 minute., motility test medium results motile: bacteria swim away from the stab line. Growth occurs throughout the tube rather than being concentrated along the line of inoculation., Most motile bacteria propel themselves by special organelles termed flagella. methods of detecting motility direct microscopic observation, direct microscopic observation types of microscopes phase contrast microscope: the bacterium and its structures appear darker than the background., Most motile bacteria propel themselves by special organelles termed flagella. flagellar arrangements peritrichous: completely surrounded by flagella, Most motile bacteria propel themselves by special organelles termed flagella. flagellar arrangements lophotrichous: two or more flagella at one or both poles of the cell, Heat-fix a smear of Bacillus megaterium to a clean glass slide. step 2 Place a piece of blotting paper over the smear and saturate with malachite green. Stain for 1 minute.
